Electron paramagnetic resonance spectroscopic studies of the electron transfer reaction of Hantzsch ester and a pyrylium salt

Sebők-Nagy K, Rózsár D, L. G. Puskás LG, Balázs Á and Páli T Abstract The oxidation of Hantzsch ester by a pyrylium cation takes place via electron–proton–electron transfer. The reaction was investigated with EPR spectroscopy using TEMPO and DMPO for inhibition and spin trapping, respectively, of the radicals appearing during the reaction. The present in-depth EPR study ...

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Synthesis and Cytoprotective Characterization of 8-Hydroxyquinoline Betti Products

Kanizsai I, Madácsi R, Hackler L Jr, Gyuris M, Szebeni GJ, Huzián O, Puskás LG. Abstract The 8-hydroxyquinoline pharmacophore scaffold has been shown to possess a range of activities as metal chelation, enzyme inhibition, cytotoxicity, and cytoprotection. Based on our previous findings we set out to optimize the scaffold for cytoprotective activity for its potential ...

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Decoding DNA labels by melting curve analysis using real-time PCR

Balog JA, Fehér LZ, Puskás LG. Abstract Synthetic DNA has been used as an authentication code for a diverse number of applications. However, existing decoding approaches are based on either DNA sequencing or the determination of DNA length variations. Here, we present a simple alternative protocol for labeling different objects using a small number of ...

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The Curcumin Analog C-150, Influencing NF-κB, UPR and Akt/Notch Pathways Has Potent Anticancer Activity in Vitro and in Vivo

Hackler L Jr, Ózsvári B, Gyuris M, Sipos P, Fábián G, Molnár E, Marton A, Faragó N, Mihály J, Nagy LI, Szénási T, Diron A, Párducz Á, Kanizsai I, Puskás LG. Abstract C-150 a Mannich-type curcumin derivative, exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of cell proliferation by C-150 ...

Read moreThe Curcumin Analog C-150, Influencing NF-κB, UPR and Akt/Notch Pathways Has Potent Anticancer Activity in Vitro and in Vivo

Facile synthesis of 1H-imidazo[1,2-b]pyrazoles via a sequential one-pot synthetic approach

Demjén A, Gyuris M, Wölfling J, Puskás LG, Kanizsai I. Abstract 5-Aminopyrazole-4-carbonitrile and ethyl 5-aminopyrazole-4-carboxylate, as potential trifunctional building blocks are introduced in a facile, chemo- and regioselective multicomponent assembly of imidazo[1,2-b]pyrazoles via the Groebke–Blackburn–Bienaymé reaction (GBB reaction). Besides the synthetic elaboration of a green-compatible isocyanide-based access in three-component mode, we describe an operationally simple, ...

Read moreFacile synthesis of 1H-imidazo[1,2-b]pyrazoles via a sequential one-pot synthetic approach

High-density real-time PCR-based in vivo toxicogenomic screen to predict organ-specific toxicity

Fabian G, Farago N, Feher LZ, Nagy LI, Kulin S, Kitajka K, Bito T, Tubak V, Katona RL, Tiszlavicz L, Puskas LG. Abstract Toxicogenomics, based on the temporal effects of drugs on gene expression, is able to predict toxic effects earlier than traditional technologies by analyzing changes in genomic biomarkers that could precede subsequent protein ...

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Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin

Molnár E, Fábián G, Klem J, Darula Z, Hunyadi-Gulyás E, Medgyesi A, Medzihradszky KF, Puskás LG. Abstract Cellular drug target identification through affinity chromatography is often hindered by the quantity of nonspecific binders, such as cytoskeletal and heat shock proteins. Thus, we prepared a 2-aminobenzimidazole-tethered depletion resin designed for removal of these proteins, and tested ...

Read moreRemoval of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin