High-density real-time PCR-based in vivo toxicogenomic screen to predict organ-specific toxicity

Fabian G, Farago N, Feher LZ, Nagy LI, Kulin S, Kitajka K, Bito T, Tubak V, Katona RL, Tiszlavicz L, Puskas LG. Abstract Toxicogenomics, based on the temporal effects of drugs on gene expression, is able to predict toxic effects earlier than traditional technologies by analyzing changes in genomic biomarkers that could precede subsequent protein translation and initiation of histological ...

Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin

Molnár E, Fábián G, Klem J, Darula Z, Hunyadi-Gulyás E, Medgyesi A, Medzihradszky KF, Puskás LG. Abstract Cellular drug target identification through affinity chromatography is often hindered by the quantity of nonspecific binders, such as cytoskeletal and heat shock proteins. Thus, we prepared a 2-aminobenzimidazole-tethered depletion resin designed for removal of these proteins, and tested it on human lung carcinoma ...