Affinity chromatography

Avicor offers an affinity chromatography service from unmodified small drug-like molecules. Our affinity chromatography service is divided into 2 steps:

Attachment test

Avicor's immobilisation chemistry is compatible with the majority of compounds (according to our findings approximately 90% of a random compound library). In the first step of our service we verify on a microarray that the compounds can be covalently bound to an Avi-ChemixTM microarray glass surface.

Affinity purification

Avi-LinkTM affinity beads are activated with Avicor's immobilisation chemistry. After activation the compound of interest is immobilised onto the affinity beads. A negative control column is produced from glass beads capped with ethanolamine.
Total protein extracts are prepared from the cell lysate of choice and processed with a protease, kinase and phosphatase inhibitor containing buffer. Proteins that bind to the resin are removed from the lysate. This lysate is brought onto the affinity column and the effluent is further divided into 5 fractions and concentrated with molecular exclusion membrane tubes. The proteins in the effluent are identified with a MALDI-TOF mass spectrometer.

Chemical Microarrays

Avicor offers companies custom Avi-ChemixTM microarrays printed from the company's proprietary chemical compound library. This allows for full integration of the Avi-ChemixTM affinity assay in existing drug discovery programs of pharmaceutical and biotech companies. Avicor can print the custom arrays from coded libraries without knowing any molecular structure. On location printing is also an option for sizable projects. These measures guaranty full confidentially.
To laboratories that lack both microarray processing infrastructure and the resources for conventional high throughput assays we provide screenings with the Avi-ChemixTM technologies as a service.
Other solutions
Please feel free to contact us with requests for more information on our technology or to discuss a custom made solution for your problem with our technology.